细粒棘球绦虫三磷酸甘油醛脱氢酶基因的克隆、表达及其分子特征的生物信息学分析

doi:10.11843/j.issn.0366-6964.2015.09.019

Abstract

Abstract:

As a key enzyme in the glycolytic pathway，glyceraldehyde-3-phosphate dehydrogenase (GAPDH) has wide applications (vaccine candidate and drug target) against some parasites，but until now，few studies have been carried out in tapeworms.In order to determine its molecular character and practical value，Echinococcus granulosus GAPDH (EgGAPDH) was cloned，expressed and analyzed.The EgGAPDH gene isolated from E.granulosus cDNA was 1 011 bp in length，encoding 336 amino acids.The quaternary structure of this protein consisted of four chemically identical subunits，O，P，Q，and R.Each subunit consisted of an NAD+-binding domain (residues 4-151 and 315-336)，a catalytic domain (residues 156-314) and one enzyme active site (residues 149-156).Recombinant EgGAPDH was expressed and purified from Escherichia coli.Specific immunogenicity of EgGAPDH was shown by Western blot，ELISA analysis，and epitope prediction (16 B cell epitopes and 9 cytotoxic T lymphocyte epitopes).EgGAPDH gene was successfully amplified，cloned and expressed，EgGAPDH has three functional areas and a number of epitope prediction，combing with the results of Western blot and ELISA，it may be serve as a vaccine candidate and drug target against larval of E.granulosus.

CLC Number:

S852.734

WANG Jia-hai，WANG Ning，HU Dan-dan，ZHONG Xiu-qin，SONG Xing-ju，GU Xiao-bin，WANG Tao，YANG Guang-you. Cloning，Expression and Bioinformatical Analysis of Echinococcus granulosus Glyceraldehyde-3-phosphate Dehydrogenase Gene[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(9): 1629-1637.

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Cloning，Expression and Bioinformatical Analysis of Echinococcus granulosus Glyceraldehyde-3-phosphate Dehydrogenase Gene

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